OneLab library

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Version 5, 26 Jun 2022 at 8:04 AM
The task of accurately separating, identifying, and quantitating amino acids in the research, development, and commercialization of food, feed, and biotherapeutic products is challenging. To analyze released amino acids by HPLC or UPLC, Waters provides many solutions that use pre-column derivatization so that amino acids can be analyzed by optical detection.
Version 1, 6 Dec 2020 at 10:26 AM
Investigating the ACE inhibitory activity of active biomolecules is a valuable source of drugs for the treatment of cardiovascular diseases. OneLab helps mitigate the effects of pipetting errors and variability during the assessment of ACE inhibition and IC50 determination. This allows for the delivery of more reliable and accurate data, while eventually accelerating biological discovery.
Version 2, 26 Jun 2022 at 8:04 AM
Developing a robust process for manufacturing therapeutic mAbs poses many analytical challenges. This requires high-throughput methods with fast sample preparation, robust analytics, and easy data interpretation. Here we present a fully automated workflow for sample preparation for middle-level analysis by LC-MS of mAbs directly from crude protein samples such as harvested cell culture fluid.
Version 3, 26 Jun 2022 at 8:04 AM
Antibody purification is routinely performed in biopharmaceutical research. Here, we present a rapid automated Protein A-based antibody purification method using magnetic beads. The robust protocol ensures high antibody recovery. The automation capabilities of the Andrew+ Pipetting Robot allow for the flexibility of processing samples up to 48 samples depending on throughput needs.
Version 3, 26 Jun 2022 at 8:04 AM
Subunit analysis of therapeutic antibodies by LC-MS using FabRICATOR (IdeS) digestion provides a rapid approach for analyzing many important quality attributes including Fc glycosylation and oxidation. Here, we describe protocols for automated sample preparation using the Andrew+ Pipetting Robot.
Version 1, 26 Jun 2022 at 8:03 AM
High-throughput analytical platforms for the characterization of proteins produced at clone selection stage have also been increasingly on demand to facilitate the development of these biopharmaceuticals. Here we present a fully automated workflow for sample preparation and LC-MS analysis of mAbs obtained directly from unrefined samples such as spent cell culture media including host cells protein.
Version 4, 27 Mar 2022 at 8:27 AM
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps. Together with Andrew Alliance, we have developed the hardware and protocols for easy automation of up to 6 plasmid midi preps in parallel, providing you with a reliable solution for plasmid purification without the tedious work of manually processing samples.
Version 1, 30 May 2021 at 8:14 AM
The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric method designed to measure the concentration of a protein of interest compared to a protein standard. OneLab guides you through the workflow and assists in setting up serial dilutions of the standard protein so as to ensure accurate estimation of your protein concentration and full traceability.
Version 2, 21 Nov 2021 at 10:01 AM
Gene Knockout is a technique that is fundamental to the investigation of gene function, which has been made easier and faster by the revolutionary breakthrough of CRISPR technology. OneLab provides clear step-by-step guidance on correct execution of the clonal isolation process to ensure optimal input, as well as identification of wells with single colonies. Additionally, it records each step in these procedures, enabling full traceability.
Version 2, 27 Mar 2022 at 8:27 AM
Real-time qPCR is widely used to detect infectious pathogens in human secretions. Preventive, control measures against 2019-nCoV critically depend on the timely and accurate diagnosis of cases. Automating the reaction preparation of the 2019-nCoV Real-Time RT-PCR Diagnostic Panel will significantly reduce pipetting errors and decreases hands-on time, ensuring more accurate and timely results.
Version 2, 27 Mar 2022 at 8:27 AM
Dilute-and-shoot is a simple and rapid analytical method for multi-analyte identification. Although sample preparation is minimal, it remains a critical step to ensure accurate quantitation. The protocols described here use the dilute-and-shoot approach and take advantage of the Andrew+ capabilities to automate sample preparation ensuring maximum pipetting precision and efficient mixing.
Version 1, 6 Dec 2020 at 10:26 AM
Library preparation aims to build a collection of DNA fragments for next-generation sequencing (NGS). A high-quality DNA library guarantees uniform and consistent genome coverage, thus delivering comprehensive and reliable sequencing data. OneLab helps you streamline and trace critical steps in your library preparation process to achieve the best possible DNA quality and purity.
Version 2, 27 Mar 2022 at 8:27 AM
Adequate endotoxin testing is invaluable in ensuring quality and safety. LAL assays require cumbersome sample preparation and are easily disturbed by different constituents of the sample. EndoLISA, combining solid-phase format and synthetic rFC, achieves sensitive detection of endotoxin by eliminating interfering components. It can be automated, which accelerates the test with minimal human error.
Version 1, 21 Feb 2021 at 10:42 AM
Circulating cell-free DNA derived from diseased cells is a promising biomarker for non-invasive diagnosis and treatment monitoring. Harnessing the potential of ccfDNA involves not only an understanding of its biology and dynamics but also the standardization of isolation methods. OneLab can act as a platform to execute your ccfDNA isolation protocol, mitigating the effect of pipetting variability.
Version 5, 26 Jun 2022 at 8:04 AM
Monitoring N-Glycosylation is increasingly applied in the development of biopharmaceuticals and the study of diseased cells. Glycan preparation is laborious, with analytical methods lacking sensitivity. GlycoWorks RapiFluor-MS accelerates N-glycan preparation, whilst improving detection sensitivity. Repeatability and traceability are assured when paired with Andrew Alliance automation solutions.
Version 2, 27 Mar 2022 at 8:28 AM
N-glycans affect the safety and efficacy of many therapeutic proteins and are routinely monitored for the quality of biotherapeutics. Here, we present a complementary method for analyzing the complex N-glycans released from human chorionic gonadotrophin (hCG). The automated method provides a reproducible glycan profile and is suitable for commercial as well as research environments.
Version 2, 30 May 2021 at 8:14 AM
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps. Using NucleoBond Xtra Maxi, yields of up to 1000 μg of ultrapure plasmid DNA can be obtained based on reliable and well-established anion exchange chromatography.
Version 2, 30 May 2021 at 8:14 AM
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps. Using NucleoBond Xtra Midi, yields of up to 500 μg of ultrapure plasmid DNA can be obtained based on reliable and well-established anion exchange chromatography.
Version 2, 26 Jun 2022 at 8:04 AM
Here we present an automation solution for the quantification of four immunosuppressive drugs in whole human blood. These four drugs are commonly used in clinical research for studying therapeutic drug monitoring. The automated method produces comparable results to the manual workflow and offers time-saving benefits with up to 96 samples prepared in less than an hour and 30 minutes.
Version 2, 27 Mar 2022 at 8:27 AM
Sandwich ELISA is the most robust and sensitive type of ELISA technique. ELISA protocols traditionally have a complex workflow that requires optimization to obtain accurate results with low analytical error. To maximize accuracy and reproducibility, OneLab can guide you through your optimized protocol to ensure consistent pipetting throughout the assay and between sample replicates.
Version 2, 6 Dec 2020 at 10:26 AM
Standardizing cell preparation for flow cytometry analysis is an effective way to ensure optimal permeabilization and staining of cells, thereby resulting in consistent fluorescence intensity and event detection rate. In line with this objective, OneLab provides valuable resources to guide sample preparation and liquid handling, ensuring the delivery of reproducible results.
Version 1, 26 Jun 2022 at 8:04 AM
To analyze amino acids in biological samples by LC-MS, Waters provides a fast reproducible solution that uses pre-column derivatization built on the AccQ•Tag reaction chemistry, with the ability to quantitate over 40 amino acids in less than 10 minutes. Waters Kairos Amino Acid Kit – High Throughput (500+) enables precise and accurate preparation of over 40 amino acids using the Andrew+ Pipetting Robot.
Version 1, 28 Mar 2021 at 9:15 AM
Accurate quantification of Illumina NGS libraries is crucial to obtaining ideal cluster density and equal sample representation in the library pool. The reliability and reproducibility of qPCR measurements depend on accurate pipetting during sample dilutions and qPCR reaction setup. OneLab helps in minimizing user-related variations in liquid handling by providing full guidance and traceability.
Version 1, 21 Feb 2021 at 10:42 AM
Simple, one-step affinity purification of recombinant proteins using magnetic beads is an attractive option to explore optimal expression and analytical conditions, thereby accelerating the translation of research findings into practice. The aim of OneLab is to streamline protein purification by integrating connected devices for magnetic separation as well as to ensure consistent pipetting.
Version 1, 30 Jan 2022 at 7:54 AM
Lipids are important molecules involved in energy metabolism, signaling, and membrane formation. They are pivotal in understanding disease and its progression. Here we highlight an automated MTBE liquid-liquid extraction method of lipids and small molecules from biological matrices. The automated method helps streamline this multi-step extraction protocol saving time and reducing operator errors.
Version 3, 25 Jul 2021 at 9:33 AM
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics. MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including genomic DNA isolation from human specimens, such as whole blood, serum, plasma, or other body fluids.
Version 1, 5 Sep 2021 at 8:37 AM
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. MACHEREY-NAGEL RNA purification kits in combination with Pipette+ guidance deliver reliably pure RNA for your demanding applications.
Version 4, 25 Jul 2021 at 9:33 AM
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics. MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including viral RNA and DNA isolation from human specimens, such as swabs or viral transport media.
Version 1, 25 Jul 2021 at 9:33 AM
MACHEREY-NAGEL kits are developed to efficiently purify high-quality DNA, especially from challenging samples. Overcome difficulties associated with efficient isolation of nucleic acids from hard-to-lyse samples using kits tailored to distinct sample types such as food, soil, stool, and plants and achieve the best possible reproducibility with Pipette+.
Version 3, 25 Jul 2021 at 9:33 AM
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics. MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including genomic DNA isolation from tissues and cells.
Version 3, 26 Jun 2022 at 8:04 AM
Mixed-mode SPE is a sample clean-up technique for LC-MS analysis. Selecting a mixed-mode SPE sorbent can be challenging, requiring a good understanding of the sorbent/analyte chemistry. In addition, it involves accurate and precise pipetting steps. This protocol demonstrates a rapid and robust Andrew+ automated mixed-mode SPE sorbent selection method using the Oasis 2x4 sorbent selection plate.
Version 1, 26 Jun 2022 at 8:03 AM
Developing a robust process for manufacturing biopharmaceuticals poses many challenges. It requires high-throughput methods with fast sample prep, reproducible analytics, and easy data interpretation. Here we present an automated sample prep workflow to compare the O-glycopeptide profiles of heavily O-glycosylated protein therapeutics using the O-protease OpeRATOR for subsequent LC-MS analysis.
Version 4, 27 Mar 2022 at 8:27 AM
Protein precipitation is the simplest form of plasma/serum preparation. However, pipetting errors and contamination during sample preparation can impair assay sensitivity, robustness, and precision. In the following work, Andrew+ was used to prepare and extract analytes from complex biological matrices, using the Ostro sample prep plate, with subsequent LC-MS/MS analysis and quantification.
Version 1, 26 Jun 2022 at 8:04 AM
Therapeutic and endogenous peptides can be difficult to extract using SPE with high reproducibility. This is often due to user errors encountered during the extraction process. Here we present an extraction protocol for peptides using the Andrew+ robot which demonstrates rapid and robust automated mixed-mode SPE sorbent selection using the Oasis Peptide Method Development 96 well μElution Plate.
Version 1, 25 Jul 2021 at 9:33 AM
Plasmid purification, PCR Clean-up and DNA extraction from gels are the most commonly used applications in molecular biology labs. Both applications are also important steps in the cloning workflow. The MACHEREY-NAGEL NucleoSpin Plasmid and NucleoSpin Gel and PCR Clean-up kit in combination with Pipette+ is the easiest way to purify isolate plasmid DNA and DNA fragments from agarose gels or PCR reactions.
Version 1, 6 Dec 2020 at 10:26 AM
Modern sequencing technology requires precise and reliable DNA sample clean-up and size-selective purification to ensure accurate and consistent data. Using the ProNex® size-selection chemistry, OneLab allows clean and error-free processing of your DNA samples with full traceability of individual pipetting actions.
Version 1, 21 Feb 2021 at 10:42 AM
The expression level of specific receptors serves as a biomarker for pathological conditions. The quantitation of their number on target cells is very informative from a diagnostic and therapeutic perspective. Consistent and repeatable sample preparation and staining of calibration beads and cells are ensured through accurate pipetting using OneLab, thereby minimizing variation in ABC assignments.
Version 1, 6 Dec 2020 at 10:26 AM
Accurate and consistent quantitation of nucleic acids is a prerequisite for high-value applications in genomics. Fluorometric quantification provides a fast, sensitive and selective measurement of the concentration of nucleic acids in your sample. OneLab enables you to record the step-by-step execution of your sample preparation, ensuring easy and error-free processing.
Version 2, 27 Mar 2022 at 8:28 AM
Peptide analysis can be a demanding process especially with the increased need for a greater understanding of SARS-CoV-2 infection. Waters SARS-CoV-2 LC-MS kit enables precise and reproducible preparation of three SARS-CoV-2 peptides from the NCAP protein using the Andrew+ pipetting robot.
Version 2, 30 Jan 2022 at 7:54 AM
The repetitive pipetting to or from wells during a plate reformatting procedure could introduce errors and variability, which is detrimental to the reliability of experimental results. Using OneLab software, Andrew Alliance automation solutions provide the best performance in time and precision, thereby making plate reformatting less time-consuming and more accurate.
Version 1, 6 Dec 2020 at 10:26 AM
Quantitative RT-PCR is a powerful tool to investigate gene expression. However, several challenges are encountered by scientists during sample preparation, including pipetting errors and DNA contamination. OneLab addresses these issues by providing step-by-step guidance for pipetting and traceability, so as to mitigate unnecessary waste of reagents and improved data reproducibility.
Version 4, 27 Mar 2022 at 8:27 AM
LC-MS analytical method development requires flexible and reproducible sample preparation for accurate and robust analyte quantification. This method provides example protocols of automated sample preparation for standard curve generation, using the Andrew+ Pipetting Robot with cloud-native OneLab software, ensuring downstream accurate and reproducible LC-MS quantification of different analytes.
Version 3, 26 Jun 2022 at 8:04 AM
The manufacturing of therapeutic IgG antibodies is a complex process that requires precise monitoring. A major issue to address in relation to IgG measurement is reproducibility. The Valita®Titer assay combined with Andrew Alliance’s automation and OneLab software provides a standardized and traceable solution for fast and accurate IgG quantitation, thus mitigating the effect of user variability.