OneLab library

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main illustration image - ACE Inhibition Assay
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
Investigating the ACE inhibitory activity of active biomolecules is a valuable source of drugs for the treatment of cardiovascular diseases. OneLab helps mitigate the effects of pipetting errors and variability during the assessment of ACE inhibition and IC50 determination. This allows for the delivery of more reliable and accurate data, while eventually accelerating biological discovery.
main illustration image - AccQ•Tag™ Amino Acid Sample Prep
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 7, 23 Jul 2023 at 8:13 AM
The task of accurately separating, identifying, and quantitating amino acids in the research, development, and commercialization of food, feed, and biotherapeutic products is challenging. To analyze released amino acids by HPLC or UPLC, Waters provides many solutions that use pre-column derivatization so that amino acids can be analyzed by optical detection.
main illustration image - Analysis of mAbs in Crude Samples
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 2, 11 Jun 2023 at 8:12 AM
Developing a robust process for manufacturing therapeutic mAbs poses many analytical challenges. This requires high-throughput methods with fast sample preparation, robust analytics, and easy data interpretation. Here we present a fully automated workflow for sample preparation for middle-level analysis by LC-MS of mAbs directly from crude protein samples such as harvested cell culture fluid.
main illustration image - Automated Antibody Purification
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 3, 2 Oct 2022 at 8:16 AM
Antibody purification is routinely performed in biopharmaceutical research. Here, we present a rapid automated Protein A-based antibody purification method using magnetic beads. The robust protocol ensures high antibody recovery. The automation capabilities of the Andrew+ Pipetting Robot allow for the flexibility of processing samples up to 48 samples depending on throughput needs.
main illustration image - Automated Bioanalytical SPE
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 10 Sep 2023 at 8:14 AM
The following work demonstrates the capabilities of the Andrew+ Pipetting Robot in combination with the Extraction+ Connected Device for fully automated bioanalytical solid phase extraction (SPE) for a variety of pharmaceutical drugs from plasma. Protocols for both cartridge and 96-well SPE plate formats are included. Calibration curves and QC samples are created in one protocol and extracted in a separate one.
main illustration image - Automated NucleoBond Xtra Midiprep
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 6, 11 Jun 2023 at 8:12 AM
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps. Together with Andrew Alliance, we have developed the hardware and protocols for easy automation of up to 6 plasmid midi preps in parallel, providing you with a reliable solution for plasmid purification without the tedious work of manually processing samples.
main illustration image - Automated Plate & Cartridge RP-SPE
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 10 Sep 2023 at 8:14 AM
This work demonstrates the capabilities of the Andrew+ Pipetting Robot configured with the Extraction+ Connected Device for fully automated SPE sample preparation using cartridge and plate formats. Described Protocols use a simple 3-step SPE procedure (load, wash, and elution) in combination with Waters reversed-phase Oasis HLB cartridges (1, 3, and 6 cc size) and 96-well macro and μElution plates.
main illustration image - Automated Protein Digestion in Food Matrix
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 3 Dec 2023 at 11:49 AM
Bottom-up proteomics is a useful approach for the discovery of signature peptides to identify specific proteins found in the complex food matrix. Here we present a fully automated sample preparation protocol for protein digestion using the ProteinWorks kit, and the final mixture obtained will be ready for a typical LCMS method.
main illustration image - Automated mAb Subunit Generation
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 3, 11 Jun 2023 at 8:12 AM
Subunit analysis of therapeutic antibodies by LC-MS using FabRICATOR (IdeS) digestion provides a rapid approach for analyzing many important quality attributes including Fc glycosylation and oxidation. Here, we describe protocols for automated sample preparation using the Andrew+ Pipetting Robot.
main illustration image - Automated mAb Subunit Screening
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 2 Oct 2022 at 8:16 AM
High-throughput analytical platforms for the characterization of proteins produced at clone selection stage have also been increasingly on demand to facilitate the development of these biopharmaceuticals. Here we present a fully automated workflow for sample preparation and LC-MS analysis of mAbs obtained directly from unrefined samples such as spent cell culture media including host cells protein.
main illustration image - Automation of PFAS Samples in Milk Matrices using SPE
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 7 Apr 2024 at 8:38 AM
The European Food Safety Authority (EFSA) predicts that PFAS exposure is primarily through food. Milk, being the staple food product for toddlers and children, would affect them disproportionately compared to adults, so there is a need to monitor PFAS levels in milk. In addition to using Andrew+ and Extraction+ for improved throughput and reduced errors, this method uses Oasis WAX SPE cartridges to enhance PFAS recovery and reduce matrix interferences for LC-MS/MS analysis.
main illustration image - BCA Colorimetric Protein Assay
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 2, 10 Sep 2023 at 8:14 AM
The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric method designed to measure the concentration of a protein of interest compared to a protein standard. OneLab guides you through the workflow and assists in setting up serial dilutions of the standard protein so as to ensure accurate estimation of your protein concentration and full traceability.
main illustration image - CRISPR/Cas9-mediated Gene Knockout
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 2, 2 Oct 2022 at 8:16 AM
Gene Knockout is a technique that is fundamental to the investigation of gene function, which has been made easier and faster by the revolutionary breakthrough of CRISPR technology. OneLab provides clear step-by-step guidance on correct execution of the clonal isolation process to ensure optimal input, as well as identification of wells with single colonies. Additionally, it records each step in these procedures, enabling full traceability.
main illustration image - DNA Sequencing Library Preparation
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
Library preparation aims to build a collection of DNA fragments for next-generation sequencing (NGS). A high-quality DNA library guarantees uniform and consistent genome coverage, thus delivering comprehensive and reliable sequencing data. OneLab helps you streamline and trace critical steps in your library preparation process to achieve the best possible DNA quality and purity.
main illustration image - Detection of 2019-nCoV by RT-qPCR
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 2, 2 Oct 2022 at 8:16 AM
Real-time qPCR is widely used to detect infectious pathogens in human secretions. Preventive, control measures against 2019-nCoV critically depend on the timely and accurate diagnosis of cases. Automating the reaction preparation of the 2019-nCoV Real-Time RT-PCR Diagnostic Panel will significantly reduce pipetting errors and decreases hands-on time, ensuring more accurate and timely results.
main illustration image - Dilute and Shoot
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 2, 30 Oct 2022 at 8:14 AM
Dilute-and-shoot is a simple and rapid analytical method for multi-analyte identification. Although sample preparation is minimal, it remains a critical step to ensure accurate quantitation. The protocols described here use the dilute-and-shoot approach and take advantage of the Andrew+ capabilities to automate sample preparation ensuring maximum pipetting precision and efficient mixing.
main illustration image - EndoLISA® Detection Assay
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 2, 2 Oct 2022 at 8:16 AM
Adequate endotoxin testing is invaluable in ensuring quality and safety. LAL assays require cumbersome sample preparation and are easily disturbed by different constituents of the sample. EndoLISA, combining solid-phase format and synthetic rFC, achieves sensitive detection of endotoxin by eliminating interfering components. It can be automated, which accelerates the test with minimal human error.
main illustration image - EpiQuik™ Circulating DNA Isolation
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
Circulating cell-free DNA derived from diseased cells is a promising biomarker for non-invasive diagnosis and treatment monitoring. Harnessing the potential of ccfDNA involves not only an understanding of its biology and dynamics but also the standardization of isolation methods. OneLab can act as a platform to execute your ccfDNA isolation protocol, mitigating the effect of pipetting variability.
main illustration image - Fungitell® Glucan Detection Assay
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 2, 10 Sep 2023 at 8:14 AM
The Fungitell® chromogenic assay is used to detect (1→3)-β-D-Glucan, a biomarker for invasive fungal disease. The Limulus Amebocyte Lysate (LAL) based assay requires a complex plate building process with numerous sample handling and pipetting steps including standard curve preparation, control testing, in-plate sample pretreatment, and reagent dispensing. These time consuming and tedious steps can be accomplished using an Andrew+ robot.
main illustration image - GlycoWorks® N-Glycan Automated Sample Prep
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 6, 7 Apr 2024 at 8:38 AM
Monitoring N-Glycosylation is increasingly applied in the development of biopharmaceuticals and the study of diseased cells. Glycan preparation is laborious, and analytical methods lack sensitivity. GlycoWorks RapiFluor-MS accelerates N-glycan preparation while improving detection sensitivity. Repeatability and traceability are assured when paired with Waters Lab automation solutions.
main illustration image - GlycoWorks® Reducing Protocol
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 2, 2 Oct 2022 at 8:16 AM
N-glycans affect the safety and efficacy of many therapeutic proteins and are routinely monitored for the quality of biotherapeutics. Here, we present a complementary method for analyzing the complex N-glycans released from human chorionic gonadotrophin (hCG). The automated method provides a reproducible glycan profile and is suitable for commercial as well as research environments.
main illustration image - Guided NucleoBond Xtra Maxiprep
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 2, 2 Oct 2022 at 8:16 AM
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps. Using NucleoBond Xtra Maxi, yields of up to 1000 μg of ultrapure plasmid DNA can be obtained based on reliable and well-established anion exchange chromatography.
main illustration image - Guided NucleoBond Xtra Midiprep
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 2, 2 Oct 2022 at 8:16 AM
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps. Using NucleoBond Xtra Midi, yields of up to 500 μg of ultrapure plasmid DNA can be obtained based on reliable and well-established anion exchange chromatography.
main illustration image - Immunosuppressant Sample Prep
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 2, 2 Oct 2022 at 8:16 AM
Here we present an automation solution for the quantification of four immunosuppressive drugs in whole human blood. These four drugs are commonly used in clinical research for studying therapeutic drug monitoring. The automated method produces comparable results to the manual workflow and offers time-saving benefits with up to 96 samples prepared in less than an hour and 30 minutes.
main illustration image - Indirect Sandwich ELISA
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 3, 10 Sep 2023 at 8:14 AM
Sandwich ELISA is the most robust and sensitive type of ELISA technique. ELISA protocols traditionally have a complex workflow that requires optimization to obtain accurate results with low analytical error. To maximize accuracy and reproducibility, OneLab can guide you through your optimized protocol to ensure consistent pipetting throughout the assay and between sample replicates.
main illustration image - Intact Mass and Cell Culture Media Workflows
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 1, 22 Oct 2023 at 8:15 AM
This protocol enables upstream engineers to routinely collect product quality attributes and metabolite information for monitoring and optimization through the use of Waters Bioprocess Walk-Up Solutions. With the OneLab waters_connect integration, Waters provides a streamlined and user-friendly process for performing precise and accurate sample preparation using the Andrew+ Pipetting Robot directly followed by acquiring high-quality LC-MS data using the BioAccord System.
main illustration image - Intracellular Flow Cytometry
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 2, 2 Oct 2022 at 8:16 AM
Standardizing cell preparation for flow cytometry analysis is an effective way to ensure optimal permeabilization and staining of cells, thereby resulting in consistent fluorescence intensity and event detection rate. In line with this objective, OneLab provides valuable resources to guide sample preparation and liquid handling, ensuring the delivery of reproducible results.
main illustration image - KAPA DNA Library Quantification
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
Accurate quantification of Illumina NGS libraries is crucial to obtaining ideal cluster density and equal sample representation in the library pool. The reliability and reproducibility of qPCR measurements depend on accurate pipetting during sample dilutions and qPCR reaction setup. OneLab helps in minimizing user-related variations in liquid handling by providing full guidance and traceability.
main illustration image - Kairos Amino Acid Sample Prep
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 2 Oct 2022 at 8:16 AM
To analyze amino acids in biological samples by LC-MS, Waters provides a fast reproducible solution that uses pre-column derivatization built on the AccQ•Tag reaction chemistry, with the ability to quantitate over 40 amino acids in less than 10 minutes. Waters Kairos Amino Acid Kit – High Throughput (500+) enables precise and accurate preparation of over 40 amino acids using the Andrew+ Pipetting Robot.
main illustration image - MTBE Lipid Extraction
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 30 Oct 2022 at 8:14 AM
Lipids are important molecules involved in energy metabolism, signaling, and membrane formation. They are pivotal in understanding disease and its progression. Here we highlight an automated MTBE liquid-liquid extraction method of lipids and small molecules from biological matrices. The automated method helps streamline this multi-step extraction protocol saving time and reducing operator errors.
main illustration image - Magnetic His-Protein Purification
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
Simple, one-step affinity purification of recombinant proteins using magnetic beads is an attractive option to explore optimal expression and analytical conditions, thereby accelerating the translation of research findings into practice. The aim of OneLab is to streamline protein purification by integrating connected devices for magnetic separation as well as to ensure consistent pipetting.
main illustration image - Matrix-Matched Pesticide Standard Curve Preparation
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 19 May 2024 at 8:17 AM
Pesticide analysis is critical to ensure agricultural commodities comply with legal limits. Matrix effects are a common concern, as they can compromise detection and quantification quality. Here, we present a fully automated protocol for matrix-matched standard curve preparation ready for a typical LC-MS/MS method.
main illustration image - NucleoSpin Blood Protocol
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 3, 2 Oct 2022 at 8:16 AM
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics. MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including genomic DNA isolation from human specimens, such as whole blood, serum, plasma, or other body fluids.
main illustration image - NucleoSpin RNA Virus Protocol
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 4, 2 Oct 2022 at 8:16 AM
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics. MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including viral RNA and DNA isolation from human specimens, such as swabs or viral transport media.
main illustration image - NucleoSpin RNA/RNA Plus
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 2 Oct 2022 at 8:16 AM
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. MACHEREY-NAGEL RNA purification kits in combination with Pipette+ guidance deliver reliably pure RNA for your demanding applications.
main illustration image - NucleoSpin Soil/Plant/Food
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 2 Oct 2022 at 8:16 AM
MACHEREY-NAGEL kits are developed to efficiently purify high-quality DNA, especially from challenging samples. Overcome difficulties associated with efficient isolation of nucleic acids from hard-to-lyse samples using kits tailored to distinct sample types such as food, soil, stool, and plants and achieve the best possible reproducibility with Pipette+.
main illustration image - NucleoSpin Tissue Protocol
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 3, 2 Oct 2022 at 8:16 AM
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics. MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including genomic DNA isolation from tissues and cells.
main illustration image - O-glycopeptide Profile Analysis
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 1, 11 Jun 2023 at 8:12 AM
Developing a robust process for manufacturing biopharmaceuticals poses many challenges. It requires high-throughput methods with fast sample prep, reproducible analytics, and easy data interpretation. Here we present an automated sample prep workflow to compare the O-glycopeptide profiles of heavily O-glycosylated protein therapeutics using the O-protease OpeRATOR for subsequent LC-MS analysis.
main illustration image - Oasis 2x4 SPE Method Development
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 4, 11 Jun 2023 at 8:12 AM
Mixed-mode SPE is a sample clean-up technique for LC-MS analysis. Selecting a mixed-mode SPE sorbent can be challenging, requiring a good understanding of the sorbent/analyte chemistry. In addition, it involves accurate and precise pipetting steps. This protocol demonstrates a rapid and robust Andrew+ automated mixed-mode SPE sorbent selection method using the Oasis 2x4 sorbent selection plate.
main illustration image - Oasis HLB SPE method development using the 20 bottle approach
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 1, 3 Dec 2023 at 11:49 AM
Solid phase extraction (SPE) is a routinely used sample preparation technique in bioanalytical/DMPK laboratories, selectively capturing the analyte or drug target while removing biomatrix components, thus improving the sensitivity, selectivity, and robustness of the analytical method. SPE is often perceived as complex and labor-intensive, requiring lengthy method optimization to achieve high analyte recovery. The 20 bottle method optimization approach described in the Oasis HLB care & use manual provides an elegant way of optimizing the organic composition of the wash and elution solvents in a single experiment, significantly simplifying the method development process.
main illustration image - OligoWorks™ Sample Pretreatment and SPE Extraction
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 1, 22 Oct 2023 at 8:15 AM
Sample preparation for therapeutic oligonucleotide (ONT) bioanalysis can be challenging, requiring extensive method development. Some workflows, like liquid-liquid extraction (LLE) can be difficult to automate, while others may use detergent-based reagents, requiring extensive washing and sample evaporation/reconstitution to make it LC-MS compatible. OligoWorks Kits are simple, standardized, flexible and automation-friendly sample preparation kits designed to enable accurate and robust LC-MS based bioanalytical quantitation across a diversity of oligonucleotides.
main illustration image - Ostro Protein Precipitation
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 4, 30 Oct 2022 at 8:14 AM
Protein precipitation is the simplest form of plasma/serum preparation. However, pipetting errors and contamination during sample preparation can impair assay sensitivity, robustness, and precision. In the following work, Andrew+ was used to prepare and extract analytes from complex biological matrices, using the Ostro sample prep plate, with subsequent LC-MS/MS analysis and quantification.
main illustration image - Peptide SPE Method Development
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 2, 11 Jun 2023 at 8:12 AM
Therapeutic and endogenous peptides can be difficult to extract using SPE with high reproducibility. This is often due to user errors encountered during the extraction process. Here we present an extraction protocol for peptides using the Andrew+ robot which demonstrates rapid and robust automated mixed-mode SPE sorbent selection using the Oasis Peptide Method Development 96 well μElution Plate.
main illustration image - PeptideWorks Tryptic Protein Digestion Sample Prep
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 3, 23 Jul 2023 at 8:13 AM
Reproducible peptide maps are valuable for the routine analysis of biotherapeutic proteins in R&D, QC, and manufacturing environments. Here, we present an automated comprehensive sample prep protocol using the PeptideWorks™ sample preparation kit and the Andrew+ Pipetting Robot. The automated method provides a reproducible protein digest and is suitable for commercial and research environments.
main illustration image - Plasmid Purification and Clean-up
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 1, 2 Oct 2022 at 8:16 AM
Plasmid purification, PCR Clean-up and DNA extraction from gels are the most commonly used applications in molecular biology labs. Both applications are also important steps in the cloning workflow. The MACHEREY-NAGEL NucleoSpin Plasmid and NucleoSpin Gel and PCR Clean-up kit in combination with Pipette+ is the easiest way to purify isolate plasmid DNA and DNA fragments from agarose gels or PCR reactions.
main illustration image - ProNex® Dual DNA Size Selection
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
Modern sequencing technology requires precise and reliable DNA sample clean-up and size-selective purification to ensure accurate and consistent data. Using the ProNex® size-selection chemistry, OneLab allows clean and error-free processing of your DNA samples with full traceability of individual pipetting actions.
main illustration image - ProteinSimple Jess/Wes/Abby Plate Chemiluminescence Pipetting Module
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 19 May 2024 at 8:17 AM
This protocol streamlines filling Bio-Techne’s Simple Western Jess plate with the Chemiluminescence assay. OneLab’s automation with Andrew+ ensures precise setup, aligning with the same time efficiency and reproducibility found with Jess. Simple Western Jess and other Compass for Simple Western offers a solution to quicker and better results than traditional Western blot and ELISA.
main illustration image - Pyrochrome® Endotoxin Detection Assay
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 2, 10 Sep 2023 at 8:14 AM
Endotoxin testing is a requirement for any injectable drug or medical device to ensure safety. Pyrochrome® is a 96 well microplate based chromogenic Limulus Amebocyte Lysate (LAL) assay that requires a complex plate building process with numerous pipetting steps. Standard curve and positive product control (PPC) preparation, sample arraying, and reagent dispensing are time consuming and tedious steps that can be accomplished using an Andrew+ robot.
main illustration image - QSC Fluorescent Antibody Titration
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
The expression level of specific receptors serves as a biomarker for pathological conditions. The quantitation of their number on target cells is very informative from a diagnostic and therapeutic perspective. Consistent and repeatable sample preparation and staining of calibration beads and cells are ensured through accurate pipetting using OneLab, thereby minimizing variation in ABC assignments.
main illustration image - Qubit™ Fluorometric Quantitation
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
Accurate and consistent quantitation of nucleic acids is a prerequisite for high-value applications in genomics. Fluorometric quantification provides a fast, sensitive and selective measurement of the concentration of nucleic acids in your sample. OneLab enables you to record the step-by-step execution of your sample preparation, ensuring easy and error-free processing.
main illustration image - SARS-CoV-2 LC-MS Kit (RUO)
Click & Execute Method
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Version 2, 2 Oct 2022 at 8:16 AM
Peptide analysis can be a demanding process especially with the increased need for a greater understanding of SARS-CoV-2 infection. Waters SARS-CoV-2 LC-MS kit enables precise and reproducible preparation of three SARS-CoV-2 peptides from the NCAP protein using the Andrew+ pipetting robot.
main illustration image - Simple Plate Reformatting
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 2, 2 Oct 2022 at 8:16 AM
The repetitive pipetting to or from wells during a plate reformatting procedure could introduce errors and variability, which is detrimental to the reliability of experimental results. Using OneLab software, Andrew Alliance automation solutions provide the best performance in time and precision, thereby making plate reformatting less time-consuming and more accurate.
main illustration image - Simple Serial Dilution Preparation
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 11 Dec 2022 at 8:20 AM
Workflows like ELISA assays, protein content determination and quantitative LC-MS analysis rely on serial dilutions to prepare calibration curves and quality control samples. This series of methods demonstrates simple 2- and 5-fold serial dilutions in 96-well plates in a row or column-wise fashion. The same script can be executed in a fully automated manner using Andrew+ or guided using Pipette+.
main illustration image - Single-Step SYBR® Green RT-qPCR
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 1, 2 Oct 2022 at 8:16 AM
Quantitative RT-PCR is a powerful tool to investigate gene expression. However, several challenges are encountered by scientists during sample preparation, including pipetting errors and DNA contamination. OneLab addresses these issues by providing step-by-step guidance for pipetting and traceability, so as to mitigate unnecessary waste of reagents and improved data reproducibility.
main illustration image - Standard Curve Generation
Tutorial Method
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Version 4, 2 Oct 2022 at 8:16 AM
LC-MS analytical method development requires flexible and reproducible sample preparation for accurate and robust analyte quantification. This method provides example protocols of automated sample preparation for standard curve generation, using the Andrew+ Pipetting Robot with cloud-native OneLab software, ensuring downstream accurate and reproducible LC-MS quantification of different analytes.
main illustration image - Valita®Titer Antibody Quantitation
Get Started Method
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Version 4, 10 Sep 2023 at 8:14 AM
The manufacturing of therapeutic IgG antibodies is a complex process that requires precise monitoring. A major issue to address in relation to IgG measurement is reproducibility. The Valita®Titer assay combined with Andrew Alliance’s automation and OneLab software provides a standardized and traceable solution for fast and accurate IgG quantitation, thus mitigating the effect of user variability.