Bioanalysis
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This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
The following work demonstrates the capabilities of the Andrew+ Pipetting Robot in combination with the Extraction+ Connected Device for fully automated bioanalytical solid phase extraction (SPE) for a variety of pharmaceutical drugs from plasma.
Protocols for both cartridge and 96-well SPE plate formats are included. Calibration curves and QC samples are created in one protocol and extracted in a separate one.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
This work demonstrates the capabilities of the Andrew+ Pipetting Robot configured with the Extraction+ Connected Device for fully automated SPE sample preparation using cartridge and plate formats.
Described Protocols use a simple 3-step SPE procedure (load, wash, and elution) in combination with Waters reversed-phase Oasis HLB cartridges (1, 3, and 6 cc size) and 96-well macro and μElution plates.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Dilute-and-shoot is a simple and rapid analytical method for multi-analyte identification. Although sample preparation is minimal, it remains a critical step to ensure accurate quantitation.
The protocols described here use the dilute-and-shoot approach and take advantage of the Andrew+ capabilities to automate sample preparation ensuring maximum pipetting precision and efficient mixing.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Mixed-mode SPE is a sample clean-up technique for LC-MS analysis. Selecting a mixed-mode SPE sorbent can be challenging, requiring a good understanding of the sorbent/analyte chemistry. In addition, it involves accurate and precise pipetting steps.
This protocol demonstrates a rapid and robust Andrew+ automated mixed-mode SPE sorbent selection method using the Oasis 2x4 sorbent selection plate.
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Solid phase extraction (SPE) is a routinely used sample preparation technique in bioanalytical/DMPK laboratories, selectively capturing the analyte or drug target while removing biomatrix components, thus improving the sensitivity, selectivity, and robustness of the analytical method. SPE is often perceived as complex and labor-intensive, requiring lengthy method optimization to achieve high analyte recovery.
The 20 bottle method optimization approach described in the Oasis HLB care & use manual provides an elegant way of optimizing the organic composition of the wash and elution solvents in a single experiment, significantly simplifying the method development process.
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Sample preparation for therapeutic oligonucleotide (ONT) bioanalysis can be challenging, requiring extensive method development. Some workflows, like liquid-liquid extraction (LLE) can be difficult to automate, while others may use detergent-based reagents, requiring extensive washing and sample evaporation/reconstitution to make it LC-MS compatible.
OligoWorks Kits are simple, standardized, flexible and automation-friendly sample preparation kits designed to enable accurate and robust LC-MS based bioanalytical quantitation across a diversity of oligonucleotides.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Protein precipitation is the simplest form of plasma/serum preparation. However, pipetting errors and contamination during sample preparation can impair assay sensitivity, robustness, and precision.
In the following work, Andrew+ was used to prepare and extract analytes from complex biological matrices, using the Ostro sample prep plate, with subsequent LC-MS/MS analysis and quantification.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Therapeutic and endogenous peptides can be difficult to extract using SPE with high reproducibility. This is often due to user errors encountered during the extraction process.
Here we present an extraction protocol for peptides using the Andrew+ robot which demonstrates rapid and robust automated mixed-mode SPE sorbent selection using the Oasis Peptide Method Development 96 well μElution Plate.