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Version 4, 27 Mar 2022 at 8:27 AM
The task of accurately separating, identifying, and quantitating amino acids in the research, development, and commercialization of food, feed, and biotherapeutic products is challenging. To analyze released amino acids by HPLC or UPLC, Waters provides many solutions that use pre-column derivatization so that amino acids can be analyzed by optical detection.
Version 1, 6 Dec 2020 at 10:26 AM
Investigating the ACE inhibitory activity of active biomolecules is a valuable source of drugs for the treatment of cardiovascular diseases. OneLab helps mitigate the effects of pipetting errors and variability during the assessment of ACE inhibition and IC50 determination. This allows for the delivery of more reliable and accurate data, while eventually accelerating biological discovery.
Version 2, 27 Mar 2022 at 8:28 AM
Developing a robust process for manufacturing therapeutic mAbs poses many analytical challenges. This requires high-throughput methods with fast sample preparation, robust analytics, and easy data interpretation. Here we present a fully automated workflow for sample preparation for middle-level analysis by LC-MS of mAbs directly from crude protein samples such as harvested cell culture fluid.
Version 3, 27 Mar 2022 at 8:28 AM
Antibody purification is routinely performed in biopharmaceutical research. Here, we present a rapid automated Protein A-based antibody purification method using magnetic beads. The robust protocol ensures high antibody recovery. The automation capabilities of the Andrew+ Pipetting Robot allow for the flexibility of processing samples up to 48 samples depending on throughput needs.
Version 2, 27 Mar 2022 at 8:28 AM
Subunit analysis of therapeutic antibodies by LC-MS using FabRICATOR (IdeS) digestion provides a rapid approach for analyzing many important quality attributes including Fc glycosylation and oxidation. Here, we describe protocols for automated sample preparation using the Andrew+ Pipetting Robot.
Version 1, 30 May 2021 at 8:14 AM
The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric method designed to measure the concentration of a protein of interest compared to a protein standard. OneLab guides you through the workflow and assists in setting up serial dilutions of the standard protein so as to ensure accurate estimation of your protein concentration and full traceability.
Version 5, 27 Mar 2022 at 8:27 AM
Monitoring N-Glycosylation is increasingly applied in the development of biopharmaceuticals and the study of diseased cells. Glycan preparation is laborious, with analytical methods lacking sensitivity. GlycoWorks RapiFluor-MS accelerates N-glycan preparation, whilst improving detection sensitivity. Repeatability and traceability are assured when paired with Andrew Alliance automation solutions.
Version 2, 27 Mar 2022 at 8:28 AM
N-glycans affect the safety and efficacy of many therapeutic proteins and are routinely monitored for the quality of biotherapeutics. Here, we present a complementary method for analyzing the complex N-glycans released from human chorionic gonadotrophin (hCG). The automated method provides a reproducible glycan profile and is suitable for commercial as well as research environments.
Version 1, 21 Nov 2021 at 10:01 AM
To analyze amino acids in biological samples by LC-MS, Waters provides a fast reproducible solution that uses pre-column derivatization built on the AccQ•Tag reaction chemistry, with the ability to quantitate over 40 amino acids in less than 10 minutes. Waters Kairos Amino Acid Kit – High Throughput (500+) enables precise and accurate preparation of over 40 amino acids using the Andrew+ Pipetting Robot.
Version 1, 21 Feb 2021 at 10:42 AM
Simple, one-step affinity purification of recombinant proteins using magnetic beads is an attractive option to explore optimal expression and analytical conditions, thereby accelerating the translation of research findings into practice. The aim of OneLab is to streamline protein purification by integrating connected devices for magnetic separation as well as to ensure consistent pipetting.
Version 1, 21 Feb 2021 at 10:42 AM
The expression level of specific receptors serves as a biomarker for pathological conditions. The quantitation of their number on target cells is very informative from a diagnostic and therapeutic perspective. Consistent and repeatable sample preparation and staining of calibration beads and cells are ensured through accurate pipetting using OneLab, thereby minimizing variation in ABC assignments.
Version 2, 27 Mar 2022 at 8:28 AM
Peptide analysis can be a demanding process especially with the increased need for a greater understanding of SARS-CoV-2 infection. Waters SARS-CoV-2 LC-MS kit enables precise and reproducible preparation of three SARS-CoV-2 peptides from the NCAP protein using the Andrew+ pipetting robot.