Genomics
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This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps.
Together with Andrew Alliance, we have developed the hardware and protocols for easy automation of up to 6 plasmid midi preps in parallel, providing you with a reliable solution for plasmid purification without the tedious work of manually processing samples.
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Gene Knockout is a technique that is fundamental to the investigation of gene function, which has been made easier and faster by the revolutionary breakthrough of CRISPR technology.
OneLab provides clear step-by-step guidance on correct execution of the clonal isolation process to ensure optimal input, as well as identification of wells with single colonies. Additionally, it records each step in these procedures, enabling full traceability.
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Library preparation aims to build a collection of DNA fragments for next-generation sequencing (NGS). A high-quality DNA library guarantees uniform and consistent genome coverage, thus delivering comprehensive and reliable sequencing data.
OneLab helps you streamline and trace critical steps in your library preparation process to achieve the best possible DNA quality and purity.
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Real-time qPCR is widely used to detect infectious pathogens in human secretions. Preventive, control measures against 2019-nCoV critically depend on the timely and accurate diagnosis of cases.
Automating the reaction preparation of the 2019-nCoV Real-Time RT-PCR Diagnostic Panel will significantly reduce pipetting errors and decreases hands-on time, ensuring more accurate and timely results.
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Circulating cell-free DNA derived from diseased cells is a promising biomarker for non-invasive diagnosis and treatment monitoring. Harnessing the potential of ccfDNA involves not only an understanding of its biology and dynamics but also the standardization of isolation methods.
OneLab can act as a platform to execute your ccfDNA isolation protocol, mitigating the effect of pipetting variability.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps.
Using NucleoBond Xtra Maxi, yields of up to 1000 μg of ultrapure plasmid DNA can be obtained based on reliable and well-established anion exchange chromatography.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps.
Using NucleoBond Xtra Midi, yields of up to 500 μg of ultrapure plasmid DNA can be obtained based on reliable and well-established anion exchange chromatography.
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Accurate quantification of Illumina NGS libraries is crucial to obtaining ideal cluster density and equal sample representation in the library pool.
The reliability and reproducibility of qPCR measurements depend on accurate pipetting during sample dilutions and qPCR reaction setup. OneLab helps in minimizing user-related variations in liquid handling by providing full guidance and traceability.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics.
MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including genomic DNA isolation from human specimens, such as whole blood, serum, plasma, or other body fluids.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics.
MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including viral RNA and DNA isolation from human specimens, such as swabs or viral transport media.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA.
MACHEREY-NAGEL RNA purification kits in combination with Pipette+ guidance deliver reliably pure RNA for your demanding applications.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
MACHEREY-NAGEL kits are developed to efficiently purify high-quality DNA, especially from challenging samples.
Overcome difficulties associated with efficient isolation of nucleic acids from hard-to-lyse samples using kits tailored to distinct sample types such as food, soil, stool, and plants and achieve the best possible reproducibility with Pipette+.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Isolation of nucleic acids from various sample materials is the basis for a large variety of downstream analysis in the field of research, applied science, and diagnostics.
MACHEREY-NAGEL provides a diverse set of optimized solutions for nucleic acid isolation including genomic DNA isolation from tissues and cells.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Plasmid purification, PCR Clean-up and DNA extraction from gels are the most commonly used applications in molecular biology labs. Both applications are also important steps in the cloning workflow.
The MACHEREY-NAGEL NucleoSpin Plasmid and NucleoSpin Gel and PCR Clean-up kit in combination with Pipette+ is the easiest way to purify isolate plasmid DNA and DNA fragments from agarose gels or PCR reactions.
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Modern sequencing technology requires precise and reliable DNA sample clean-up and size-selective purification to ensure accurate and consistent data.
Using the ProNex® size-selection chemistry, OneLab allows clean and error-free processing of your DNA samples with full traceability of individual pipetting actions.
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Accurate and consistent quantitation of nucleic acids is a prerequisite for high-value applications in genomics. Fluorometric quantification provides a fast, sensitive and selective measurement of the concentration of nucleic acids in your sample.
OneLab enables you to record the step-by-step execution of your sample preparation, ensuring easy and error-free processing.
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Peptide analysis can be a demanding process especially with the increased need for a greater understanding of SARS-CoV-2 infection.
Waters SARS-CoV-2 LC-MS kit enables precise and reproducible preparation of three SARS-CoV-2 peptides from the NCAP protein using the Andrew+ pipetting robot.
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Quantitative RT-PCR is a powerful tool to investigate gene expression. However, several challenges are encountered by scientists during sample preparation, including pipetting errors and DNA contamination.
OneLab addresses these issues by providing step-by-step guidance for pipetting and traceability, so as to mitigate unnecessary waste of reagents and improved data reproducibility.
