Automated Bioanalytical SPE
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Overview
Fully Automated Bioanalytical SPE Sample Preparation, using the Extraction+ Connected Device with the Andrew+ Pipetting Robot
Sample preparation, which isolates target analytes from the matrix and removes as many interferences as possible, is a critical component of the bioanalytical workflow (Figure 1). Introducing automation to the bioanalytical lab helps to minimize or eliminate some of these challenges. In this application, the Andrew+ Pipetting Robot was used to generate calibration curves and QC samples using a diversity of small molecule pharmaceutical drugs in plasma. The Andrew+ Pipetting Robot (Andrew+) and the new Extraction+ Connected Device were then used to perform solid phase extraction (SPE) of these prepared samples in both 1 cc cartridge and 96-well macro plate formats.
Figure 1: Graphical illustration of the automated SPE sample extraction and LC-MS analysis workflow using the Oasis HLB SPE macro plate and 1 cc cartridges on the Andrew+ Pipetting Robot configured with the Extraction+ Connected Device.
The photo below displays the Andrew+ Pipetting Robot configured with the Extraction+ system along with labware Dominos, Andrew Alliance Bluetooth electronic pipettes and a plate gripper on the tool stand. the Extraction+ (#5) includes the connected vacuum pump, flow-through waste container, the vacuum manifold with the collar, and the integrated collar lifter. Here in this photo, as an example, 6 cc SPE cartridges are loaded into a specific 12-position adaptor and placed onto the Extraction+ manifold.
Figure 2: Photo of the Andrew+ system configured with the connected Extracton+ Manifold showing components necessary to automate a standard SPE protocol using as an example 6 cc cartridges.
Protocols and their corresponding visual Andrew+ deck layouts used for the bioanalytical plate and cartridge SPE sample preparation are described below. For the SPE plate format, a single protocol was used for sample preparation followed by two separate tutorial protocols for SPE extraction performed with Andrew+ configured with Extraction+ in which samples were either processed row or column-wise. For the SPE 1 cc cartridge format, a single protocol was used for sample preparation followed by one separate protocol for SPE extraction performed with Andrew+ configured with Extraction+.
◼️ Protocol 1 | Calibrators and QC Preparation for Macro Plate Extraction | Andrew+
ORDERING INFORMATION
Andrew+ System Components: Dominos, Devices, Electronic Pipettes & Tips
➤ Calibrators and QC Preparation for Macro Plate Extraction Protocol – Andrew+
- 2x Tip Insertion System Domino | p/n 186009612
- 1x Microtube Domino | p/n 186009601
- 1x Deepwell Microplate Domino | p/n 186009597
- 1x Storage Plate Domino | p/n 186009596
- Andrew Alliance Bluetooth Electronic Pipette, 1-ch 300 μL | p/n 186009606
- Andrew Alliance Bluetooth Electronic Pipette, 1-ch 1000 μL | p/n 186009766
- Sartorius, Optifit Tips, 5-350 μL (x21) | p/n 700013297
- Sartorius, Optifit Tips, 10-1000 μL (x48) | p/n 700013298
Consumables
- Eppendorf 2 mL Safe-Lock tube | p/n 0030120094
- Agilent 6-column reagent reservoir | p/n 201284-100
- Waters 2 mL 96-square well collection plate, cut corner H1 | p/n 186002482
◼️ Protocols 2 & 3 | Automated SPE using Oasis HLB 96-well Plate | Andrew+ Configured with Extraction+
ORDERING INFORMATION
Andrew+ System Components: Dominos, Devices, Electronic Pipettes & Tips
➤ Automated SPE using Oasis HLB 96-well Plate (by Row or by Column) Protocol – Andrew+
- 3x Tip Insertion System Domino | p/n 186009612
┉
Depending on Domino availability, Selection 1:
- 1x Deepwell Microplate | p/n 186009597
- 1x Storage Plate Domino | p/n 186009596
- Extraction+ Base Kit with Plate Gripper | p/n 176005201
- Extraction+ Plate Kit including 1x Deepwell Microplate Domino and 1x Storage Plate Domino| p/n 176005203
OR Selection 2:
- 2x Storage Plate Domino | p/n 186009596
- Extraction+ Base Kit with Plate Gripper | p/n 176005201
- Extraction+ Plate Kit including 1x Deepwell Microplate Domino and 1x Storage Plate Domino| p/n 176005203
┉
- Andrew Alliance Bluetooth Electronic Pipette, 1-ch 300 μL | p/n 186009606
- Andrew Alliance Bluetooth Electronic Pipette, 1-ch 1000 μL | p/n 186009766
┉
Protocol by Row:
- Sartorius, Optifit Tips, 5-350 μL (x2) | p/n 700013297
- Sartorius, Optifit Tips, 10-1000 μL (x111) | p/n 700013298
OR Protocol by Column:
- Sartorius, Optifit Tips, 5-350 μL (x2) | p/n 700013297
- Sartorius, Optifit Tips, 10-1000 μL (x26) | p/n 700013298
Consumables
- Agilent 6-column reagent reservoir | p/n 201284-100
- Waters Oasis HLB 96-well plate, 30 mg sorbent/well, 30 μm | p/n WAT058951
- Waters 2 mL 96-square well collection plate, cut corner H1 | p/n 186002482
- Waters polypropylene cap mat for 96-well plate, square wells | p/n 186002484
◼️ Protocol 4 | Calibrators and QC Preparation for 1 cc Cartridge Extraction | Andrew+
ORDERING INFORMATION
Andrew+ System Components: Dominos, Devices, Electronic Pipettes & Tips
➤ Calibrators and QC Preparation for 1 cc Cartridge Extraction Protocol – Andrew+
- 2x Tip Insertion System Domino | p/n 186009612
- 1x Microtube Domino | p/n 186009601
- 1x Deepwell Microplate Domino | p/n 186009597
- 1x Storage Plate Domino | p/n 186009596
- Andrew Alliance Bluetooth Electronic Pipette, 1-ch 300 μL | p/n 186009606
- Andrew Alliance Bluetooth Electronic Pipette, 1-ch 1000 μL | p/n 186009766
- Sartorius, Optifit Tips, 5-350 μL (x23) | p/n 700013297
- Sartorius, Optifit Tips, 10-1000 μL (x1) | p/n 700013298
Consumables
- Eppendorf 2 mL Safe-Lock tube | p/n 0030120094
- Agilent 6-column reagent reservoir | p/n 201284-100
- Waters 2 mL 96-square well collection plate, cut corner H1 | p/n 186002482
◼️ Protocol 5 | Automated SPE using Oasis HLB 1 cc Cartridges | Andrew+ Configured with Extraction+
ORDERING INFORMATION
Andrew+ System Components: Dominos, Devices, Electronic Pipettes & Tips
➤ Automated SPE using Oasis HLB 1 cc Cartridges Protocol – Andrew+
- 3x Tip Insertion System Domino | p/n 186009612
- 1x Deepwell Microplate | p/n 186009597
- 2x Storage plate | p/n 186009596
- Extraction+ Base Kit with Plate Gripper | p/n 176005201
- Extraction+ 1 cc Cartridge Kit including 1x Collection Labware Rack Domino| p/n 176005204
- Andrew Alliance Bluetooth Electronic Pipette, 1-ch 300 μL | p/n 186009606
- Andrew Alliance Bluetooth Electronic Pipette, 1-ch 1000 μL | p/n 186009766
- Andrew Alliance Bluetooth Electronic Pipette, 8-ch 1200 μL | p/n 186009615
- Sartorius, Optifit Tips, 5-350 μL (x2) | p/n 700013297
- Sartorius, Optifit Tips, 10-1000 μL (x12) | p/n 700013298
- Sartorius, Optifit Tips, 50-1200 μL (x48) | p/n 700013300
Consumables
- Agilent 6-column reagent reservoir | p/n 201284-100
- Waters Oasis HLB 1 cc Vac cartridge, 30 mg sorbent, 30 μm | p/n WAT094225 (NOTE: Oasis HLB 1 cc flangeless Vac cartridge, 30 mg sorbent, 30 μm with p/n 186001879, is an alternative. Cartridges with flange and flangeless cartridges work similarly)
- Waters 2 mL 96-square well collection plate, cut corner H1 | p/n 186002482
- Waters 2 mL 12x32 mm LC/GC screw-top vial | p/n 186000272C
Find more information about Recommended Pretreatment, Wash, and Elution Solutions in the:
Protocols
Contact info
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 