Qubit™ Fluorometric QuantitationGenomics
In contrast to UV/visible absorbance, fluorescence detection using the Qubit fluorometer enables specific, sensitive measurement of nucleic acid quantity and quality. It is an easy-to-apply, mix-and-read method (Figure 1) that uses small sample volumes in the microliter range (1-20 μl), which is particularly useful in cases where only small amounts of material are available. Moreover, it is an ideal technique to quantify diluted, precious samples that are used in demanding applications, such as NGS, genotyping, RNA analysis and quantitative RT-PCR. Dyes bind selectively to the biomolecules of interest and the intensity of emitted fluorescence directly reflects the amount of DNA or RNA molecules in the sample.
Figure 1: Qubit standard/sample preparation workflow. Image extracted from Quick Reference Qubit Assays PDF file (Pub. No. MAN0017210), Thermo Fisher.
View the demonstration video to learn more about the Qubit fluorometer capabilities and how to accurately measure dsDNA concentration.