This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
The Fungitell® chromogenic assay is used to detect (1→3)-β-D-Glucan, a biomarker for invasive fungal disease. The Limulus Amebocyte Lysate (LAL) based assay requires a complex plate building process with numerous sample handling and pipetting steps including standard curve preparation, control testing, in-plate sample pretreatment, and reagent dispensing. These time consuming and tedious steps can be accomplished using an Andrew+ robot.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Here we present an automation solution for the quantification of four immunosuppressive drugs in whole human blood. These four drugs are commonly used in clinical research for studying therapeutic drug monitoring. The automated method produces comparable results to the manual workflow and offers time-saving benefits with up to 96 samples prepared in less than an hour and 30 minutes.