EpiQuik™ Circulating DNA Isolation
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Published by
Andrew Alliance
Version 1, 2 Oct 2022 at 8:16 AM
Circulating cell-free DNA derived from diseased cells is a promising biomarker for non-invasive diagnosis and treatment monitoring. Harnessing the potential of ccfDNA involves not only an understanding of its biology and dynamics but also the standardization of isolation methods.
OneLab can act as a platform to execute your ccfDNA isolation protocol, mitigating the effect of pipetting variability.
Use in OneLabPlease note that this is a “Get Started Method”
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Protocols
2
Capture of Circulating Cell-Free DNA
This example protocol aims to capture and isolate nucleosomal DNA molecules in eight different plasma samples using magnetic ccfDNA capture beads.
Purification of Circulating Cell-Free DNA
This protocol allows for the purification of isolated ccfDNA molecules using MQ binding beads through repetitive washing.
Contact info
Published by
Andrew Alliance
Reproducibility and traceability for your laboratory, starting from serial dilution design. Connected devices for your lab, allowing you to run daily experiments with effiiciency. affordable automation. Error-free pipetting. Save your time. save your health.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 