General sample prep

Name
Published by
Version 1, 28 Mar 2021 at 9:15 AM
Dilute-and-shoot is a simple and rapid analytical method for multi-analyte identification. Although sample preparation is minimal, it remains a critical step to ensure accurate quantitation. The protocols described here use the dilute-and-shoot approach and take advantage of the Andrew+ capabilities to automate sample preparation ensuring maximum pipetting precision and efficient mixing.
Version 1, 25 Jul 2021 at 9:33 AM
Protein precipitation is the simplest form of plasma/serum preparation. However, pipetting errors and contamination during sample preparation can impair assay sensitivity, robustness, and precision. In the following work, Andrew+ was used to prepare and extract analytes from complex biological matrices, using the Ostro sample prep plate, with subsequent LC-MS/MS analysis and quantification.
Version 2, 25 Jul 2021 at 9:33 AM
The repetitive pipetting to or from wells during a plate reformatting procedure could introduce errors and variability, which is detrimental to the reliability of experimental results. Using OneLab software, Andrew Alliance automation solutions provide the best performance in time and precision, thereby making plate reformatting less time-consuming and more accurate.
Version 3, 10 Oct 2021 at 8:20 AM
LC-MS analytical method development requires flexible and reproducible sample preparation for accurate and robust analyte quantification. This method provides example protocols of automated sample preparation for standard curve generation, using the Andrew+ Pipetting Robot with cloud-native OneLab software, ensuring downstream accurate and reproducible LC-MS quantification of different analytes.