Standard Curve PreparationGeneral sample prep
Addressing the Challenges of Standard Curve Preparation
The preparation of samples is often the most time-consuming, but critical step, for the laboratory analyst. Any errors made in the sample preparation step will follow through the rest of the analysis potentially causing high variability in performance and in extreme cases failed analysis. In addition, the development, optimization, and execution of these assays can prove to be time-consuming and difficult to transfer between scientists and laboratories (1).
The creation of calibration standards and quality control (QC) samples is a requirement in all quantitative analyses. These samples are generated using the basic technique of sequential (or serial) dilution of the analyte(s) from a concentrated solution(s). While often not difficult, preparation of these samples, is repetitive and time-consuming, requiring consistent preparation to ensure the reliable performance of the analytical method. This makes it ideal for incorporating lab automation for this task. This allows the analyst to do other tasks, streamlines the sample preparation process, reduces the potential of human error, and ensures consistent analytical method performance.
- Accurate, precise, and robust analytic quantification performance
- Ease-of-use and time savings
- Cost savings through increased efficiency and failure reduction
- Less repetitive pipetting
- Automated method transferability
◼️ PROTOCOL 1 | Standard Curve Preparation - Nitrosamine Impurities | Andrew+
The following work highlights the development of an automated sample preparation method using the Andrew+ Pipetting Robot for the LC-MS quantification of N-nitrosamine impurities and demonstrates its performance. Due to their carcinogenicity and incidence in a variety of commodities and pharmaceuticals, robust and reliable sample preparation and analysis for their routine quantification during and after drug development and manufacturing are required.
SYSTEMS & CONSUMABLES
- Waters 0.7 mL 96-round well collection plate | p/n 186005837
- Agilent 6-column reagent reservoir | p/n 201284-100
- Fisherbrand™ Premium 1.5 mL microtube | p/n 11926955
- Pre-slit Silicone/PTFE cap mat for 96-well plates | p/n 186006332
- ACQUITY UPLC HSS T3 Column | p/n 186003539
- ACQUITY UPLC I-Class PLUS System
- Xevo TQ-XS Tandem Quadrupole Mass Spectrometer
Figure 1: Representative Nitrosamine Impurity Standard Calibration Curves (NDMA, NDEA, and NDBA), using Andrew+ Pipetting Robot for Calibration Curve Generation.
(1) Christler, A., Felföldi, E., Mosor, M., Sauer, D., Walch, N., Dürauer, A., & Jungbauer, A. (2020). Semi-automation of process analytics reduces operator effect. Bioprocess and Biosystems Engineering, 43(5), 753–764.